Program Overview
It covers sample preparation, label selection, and commonly used techniques like epifluorescence and confocal microscopy. Students gain hands-on experience through practical sessions and apply their knowledge to biological questions. This module lays the groundwork for further studies in cardiovascular sciences and metabolism and systems research.
Program Outline
Outline:
The module focuses on the fundamental principles of light microscopy and their application in commonly used microscopy techniques. It covers both theoretical and practical aspects of microscopy, including:
- Fundamental principles of microscopy: Brightfield, fluorescence, and electron microscopy.
- Sample preparation: Techniques for preparing biological samples for microscopy.
- Choice of labels and fixation: Selecting appropriate labels and fixation methods for different samples.
- Commonly used techniques: Epifluorescence and confocal microscopy.
- Application to biological samples and questions: Understanding how microscopy techniques can be used to address biological questions.
- Advanced imaging techniques: Introduction to advanced microscopy techniques.
Assessment:
The module is assessed through:
- Unseen examination (50%): A 2-hour examination covering the principles of microscopy, fluorescence, and the application of these principles in fluorescence microscopy.
- Essay (50%): A 1500-word essay that critically appraises the literature on the application of fluorescence microscopy to biological questions.
Teaching:
The module is delivered through:
- Lectures: Covering the theoretical aspects of microscopy.
- Practical sessions: Giving students hands-on experience with microscopy techniques.
- Online formats: Utilizing online resources for supplementary learning.
Other:
The module is part of a postgraduate program in the Institute of Cardiovascular Sciences and the Institute of Metabolism and Systems Research at the University of Birmingham. It provides the foundation for subsequent modules in the program and prepares students for lab-based research projects.
