Animal Cell Culture, Microcredential, Part-time

Program Overview

This hands-on program provides comprehensive training in animal cell culture techniques, including cell handling, gene transfer, and cell-based assays. With a small trainee-to-instructor ratio, participants receive personalized feedback and access to specialized equipment. The program prepares individuals for careers in biotechnology, pharmaceutical research, and other fields requiring expertise in cell culture.

Program Outline

Outline:

• Week 1 (XBIO 4010):
• Introduction to animal cell culture lab and equipment operation
• Sub-culturing adherent and suspension cells
• Cell counting and viability determination
• Thaw and cryo-preservation of cell stocks
• Gene transfer (transfection) experiment
• Classroom topics:
• Types of animal cells
• Growth media and supplements
• Microbial contamination prevention and detection
• Gene transfer and analysis techniques
• Week 2 (XBIO 4020):
• Continued sub-culturing of cells
• Preparation of cells in 96-well plates
• ELISA and cell proliferation assay
• Classroom topics:
• Cell-based assays

Teaching:

• Hands-on laboratory-based learning in the dedicated cell culture lab